This red wine mother of vinegar is perfect for making up to 1 quart (32 oz.) of red wine vinegar. Directions: Add 8 oz. of mother of vinegar and 8 oz. of water to 16 oz. of red wine to make 32 oz. (1 qt.) of vinegar. Place in glass, wood, or food safe HDPE plastic with a removable top or holes allowing for aeration. Cover holes or open top with cheesecloth or gauze to prevent insect infestation. Allow to sit 6-12 weeks. Best growth is in temperatures of 80-85 degrees Fahrenheit. You can reuse the new mother that grows on the top of your vinegar